Efek Pemberian Minyak Buah Merah (Pandanus conoideus Lam) terhadap Pertumbuhan In Vivo Kelenjar Susu Mencit C3H
Efek Pemberian Minyak Buah Merah (Pandanus conoideus Lam) terhadap Pertumbuhan In Vivo Kelenjar Susu Mencit C3H: Tinjauan Khusus Aktivcitas Proliferasi dan Apoptotis, Hening Pujasari, Puspita Eka Wuyung, Ria Kodariah, Vol 6, No 4 (2008), Journal Alam Indonesia
Breast cancer is reported as the second largest case, after cervical, of total cancer cases in Indonesian women. Although several conventional therapies for breast cancer are available such as surgery, cytostatic therapy and radiotherapy, there are times when those treatments could not give good result in a desirable patient’s recovery. Moreover, in the situation when patients are not able to pay the conventional treatments, it is common that those patients seek alternative therapies. Red Papua (Pandanus conoideus Lam.), one of Indonesian natural properties from Papua province, has been taken as daily food. Although people believe that Red Papua Oil (RPO) can be used to treat cancer, including breast cancer, the scientific evidence proving the anti-cancer effect of RPO is still very limited. It is important that there is a need to investigate whether or not oral intake of RPO inhibit proliferation activity and cancer growth, and induce apoptosis activity of mammary gland tumor of C3H strain mice.
Forty mice were randomly divided into four groups, three groups of RPO-treated and one control group. After tumor was transplanted to all of the mice, three doses of RPO (0.05 ml, 0.1 ml and 0.2 ml) were given orally to the three treated-groups once a day for twenty eight days. The tumor volume was measured every three days. By the twenty ninth day, the mice were euthanised and tumors were taken. The volume and weight of tumor were measured and macroscopically preparations were made. TUNEL essay was used to detect apoptosis activity. The proliferation activity was assessed using AgNOR. Inferential statistic ANOVA and Kruskal-Wallis were conducted to determine significance. It was found that there were no significant differences in the tumor volume, tumor weight and apoptosis index among treated and control groups; however there was a tendency to increased apoptosis activity on the treated groups. On the contrary, there were differences on the AgNOR values among the treated and control groups. These differences were statistically significant (p<0.05). It can be concluded that there was an inhibition of proliferation activity on RPO-treated mice. However, those three doses were noaat able to induce apoptosis activity and to reduce the tumor growth.